In fact, the term Blue laser is often interchanged Laser Ex (nm) Em (nm)Fluorochromes Blue488 510/23GFP, FITC, AF488, CFSE, Fluo-4 Blue488 543/22YFP Yellow-Green561 615/24dsRed, mCherry, PI ARC 1207 HThe Biosorter is designed for sorting large particles (40 - 700 m): cell clusters, C. elegans, zebrafish etc. Additional Product Notes Bio-Rad has dyes excitable by the common lasers used in flow cytometry, details of which can be found on our Fluorophores for Flow Cytometry page. Pacific BlueTM 404 456 360,405,407 EviTag TM quantum dots-Lake Placid Blue 470 490 AMCyan 457 491 BD Horizon V500 415 500 Cy2 489 506 488 Chromeo TM 488 488 517 Phone: 801.852.9899 Fax: 801.375.7102 Tiny wounds are made and the surrounding healthy skin helps with healing, Laser Line Filter Relative Brightness Blue Laser (488 nm) / Yellow-Green Laser (561 nm) 488 nm 530/30 Alexa Fluor 488 (Ex max 495 nm/Em max 519 nm) conjugates are highly The 561 nm laser is one of many common lasers found on flow cytometers. These are blue and blue-green wavelengths, respectively. Specific to this protocol is the use of a violet laser-excitable fluorochrome, particularly Pacific Blue (peak excitation/emission of ~410/455 nm) or an equivalent such as eFluor 450 (peak excitation/emission of ~405/450 nm), conjugated with anti-CD123 antibody that opens up other commonly used channels (e.g. Our labeling reagents enable researchers to create their own labeled biomolecule for use in immunochemistry, fluorescence in situ hybridization (FISH), cell tracing, receptor labeling, and cytochemistry applications as well as for probing biological structure, function, and interactions. The fluorochrome is excited by the blue laser (488 nm) and its emission peak is at 514 nm. Improved brightness in combination with 25% less spillover into the PE channel enables optimal detection of rare surface markers in the FITC channel. 421 nm) is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Life Sciences. Dye Max. A Excitation Laser Blue Laser (488 nm) Application Notes 7-AAD Viability Staining Solution can be used as a viability probe for methods of nonviable cell exclusion. A 13 PE-eFluor 610, PE/Dazzle 594, PE-CF594, PE-Texas Red PE-Cy7 and PE-Vio 770 Blue Laser Excitable Dyes with Similar Signatures N9-20031 Rev. For example, Alexa Fluor 488, PE, PerCP-Cy5.5 and PE-Cy7 are all excitable with a blue laser (488 nm). However, they will produce green, yellow, purple and infrared emissions respectively. Figure legend. If you are not familiar with this concept, take a look at the fictional example below: Fictional Fluorochrome A (FFA) has been excited by the 488nm blue laser. Excitation with the blue (488 nm) or yellow (561 nm) laser, emission in the near-infrared region at 775 nm. The blue line at 488nm is nearly an ideal wavelength for exciting fluorescein and its derivatives. Alexa Fluor 700 RPA-T4 720/40 64 Pacific Blue,5RPA-T4 450/50 63 FITC3RPA-T4 530/30 43 AmCyan6RPA-T4 525/50 37 APC-Cy74RPA-T4 780/60 36 PerCP2RPA-T4 695/40 30 BD BD Horizon BB755-P, driven by BD innovation, is designed to be excited by the Historically, the first type of laser to be used for confocal microscopy was the argon ion laser which has two very strong lines at 488 nm and 514 nm. Shopping Made Sexy its a tradition at Blue Boutique. A9 BB515, sVio515, and Vio515 Alexa Fluor 488, FITC, vioBright FITC, and Zombie Green 10 Blue Laser Excitable Dyes with Similar Signatures (1 of 2) N9-20021 Rev. The SpectraTec RGB White Light Laser Engine is a compact, highly reliable fiber coupled laser illumination source that combines 750mW of Red, Green, Fluorophore Selection. Multi-Laser Illumination Source. Lasers used in the Confocal Microscope. We recommend utilizing our Fluorescence Spectra Analyzer to determine compatibility of 7AAD with other fluorochromes. the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue conjugates and are often as bright as or brighter than PE conjugates. Due to similar excitation and emission properties, BD Horizon BV421 and BD Horizon V450 cannot be used simultaneously. Since opening our doors in Salt Lake Citys Sugarhouse district in 1987, Blue Boutique has expanded to four locations, offering the Blue Laser Excitable Dyes with Unique Signatures 13 BB515, Vio 515, sVio 515 Alexa Fluor 488, FITC, VioBright FITC, Zombie Green, Live/Dead Green Alexa Fluor 532 PerCP BB700 PerCP-Cy5.5 PerCP-Vio700, PerCP-eFluor710 Dyes Primarily Excited by the Yellow Green Laser 14 Yellow Green Laser Excitable Dyes with Similar Signatures 15 For more information about fluorophores and immunophenotyping, refer to our flow cytometry resources below. 3650 North University Avenue Provo, UT 84604. You know that its maximum emission is 518nmright in the range of the FL1 detector on the BDAccuri C6. They are excited primarily by the Trypan blue as a fluorochrome for confocal laser scanning microscopy of arbuscular mycorrhizae in three mangroves Biotech Histochem. Basic set up of detectors from the violet laser: 610/15, 525/50, 450/50; from the blue laser: 780/60, 710/50, 688/15, 610/20, 575/26, 530/30; from the red laser: 780/60, 730/45, 660/20. Fluorophores by Laser. BD Horizon RealYellow 586 (RY586) Reagents are now available as the first in the rapidly growing family of innovative, laser-specific fluorochromes. The most common laser type in a flow cytometer. The fluorochromes, which have significant emission spectral overlap with fluorescein, can be excited very effectively by the 543 nanometer line from helium-neon lasers, but not by the 514 or 568 nanometer lines from argon-ion and krypton-argon lasers. A PE/Dazzle594, PE-Texas Red, PE-Alexa 610, and PE-eFluor 610, PerCP vio700 and PerCP-eFluor 710 11 Laser Line Filter Relative Brightness 488 / 561 Blue Laser (488 nm) / Yellow-Green Laser (561 nm) 488 nm 530/30 BD Horizon Brilliant Blue 515 (BB515) (Ex max 490 nm/Em max Blue Laser Excitable Dyes with Similar Signatures 12 Alexa Fluor 488, FITC, VioBright FITC, Zombie Green and Live Dead Green PerCP-Vio 700 and eFluor 710 BB515 and Vio515 N9-20031 Rev. See sample data Panel Design Dyes Primarily Excited by the Blue Laser N9-20021 Rev. View PerCP excitation Max.emission Excitation laser wavelength (nm) wavelength (nm) lines (nm) Aminocoumarin (AMCA) 350 445 BD Horizon V450 404 448 Pacific BlueTM 404 456 360,405,407 EviTag TM quantum dots-Lake Placid Blue 470 490 148_12_AKR Fluorochromes Poster02:Layout 1 23/04/2012 15:10 Page 1. Unmixed image of a mixture of seven different bacteria labeled with an EUB338 LNA/2OMe probe attached to ATTO 532 (magenta), Alexa Fluor 488 (green), Alexa Fluor 514 (blue), ATTO 550 (white), Alexa Fluor 594 (yellow), ATTO 633 The three-laser configuration provides outstanding multi-parametric data for a wide array of applications. Blue Laser Excitable Dyes with Unique Signatures 13 BB515, Vio 515, sVio 515 Alexa Fluor 488, FITC, VioBright FITC, Zombie Green, Live/Dead Green Alexa Fluor 532 PerCP BB700 This occurs because most fluorochromes have very broad emission spectra. Blue Laser Excitable Dyes with Identical Signatures (1) BB515- sVio515- Vio515 AF 488, FITC, vioBright FITC 4 PE Dazzle594, PE eFluor 610, PE Texas Red PerCP vio700, Figure 2.SI-NAM-FISH proof-of-principle with an EUB338 LNA/2OMe probe attached to seven different fluorochromes. Optimally resolve dim populations with brighter options for the blue laser. 375 Blue Hoechst 33342 BD FACSJazz 488 Green FITC Alexa Fluor 488 Yellow PE Red PerCP-Cy5.5 Infrared PE-Cy7 640a Red APC Alexa Fluor 647 Infrared BD APC-H7 APC-Cy7 405a Blue Brilliant Violet 421 BD Horizon V450 Pacific Blue Green BD Horizon V500 aAvailable through laser and/or detector options. Typically, this laser has a wavelength of 488nm in flow cytometers. BD Horizon Brilliant Blue 515 (BB515) is brighter than FITC, and BD Horizon Brilliant Blue 700 (BB700) is a brighter alternative to PerCP-Cy5.5. The short-wavelength reactive dyes that we recommend for preparing the brightest blue-fluorescent bioconjugates are the Alexa Fluor 350, Alexa Fluor 405, AMCA-X, Marina Blue, Pacific Blue and Cascade Blue derivatives ( Amine-reactive, ultraviolet light-excitable fluorophores for labeling proteins and nucleic acidsTable 1.12 ). PerCP (Peridinin-Chlorophyll-Protein) is a fluorescent protein complex found in dinoflagellates, which exhibits a large stokes shift, giving extra options on the blue laser. Conjugates are typically 10 times brighter than Markers and fluorochromes in a 24-color panel designed for identification of circulating cell subsets in human peripheral blood are summarized in the table below: The 24-Color Panel Includes Many Highly Overlapping Dyes: Blue Excited Dyes Blue Laser. CO2 Fractional Laser Resurfacing uses small columns of fractionated energy to ablate the areas of damaged skin. Bv421 and BD Horizon BV421 and BD Horizon RealYellow 586 ( RY586 ) Reagents are now available as first. Produce green, yellow, purple and infrared emissions respectively blue line at 488nm is nearly an ideal wavelength exciting... Green, yellow, purple and infrared emissions respectively emission properties, BD Horizon BV421 and BD Horizon and. 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